Journal: BMC Genomics
Article Title: A transcript profiling approach reveals the zinc finger transcription factor ZNF191 is a pleiotropic factor
doi: 10.1186/1471-2164-10-241
Figure Lengend Snippet: Quantitative real-time PCR confirmation of the microarray results . qPCR was performed on 21 genes that showed differential regulation in response to ZNF191 overexpression and knockdown by siRNA. Gene expression levels are shown as the mean normalized to the expression of the housekeeping gene GAPDH . Each sample was measured in triplicate. Columns , mean of three independent experiments; bars , SD. Comparison of fold change produced by microarray with relative expression ratio obtained from real-time PCR, with good concordance.
Article Snippet: Microarrays were scanned with a dynamic autofocus microarray scanner (Agilent dual laser DNA microarray scanner, Agilent technologies, Palo Alto, CA, USA), using Agilent-provided parameters (Red and Green PMT were each set at 100%, and scan resolution was set to 10 μm).
Techniques: Real-time Polymerase Chain Reaction, Microarray, Over Expression, Expressing, Produced